COURSE OVERVIEW
Who is this course for?
This material is primarily aimed at analysts and organisations currently working with small molecules or performing limited testing on biomolecules. It is of limited value to analysts with high levels of experience with biomolecules.
Previous Knowledge
Participants should already have a good understanding of HPLC.
What you will learn
- Overview of the different types of biomolecules
- Biotechnology and the production of proteins
- SEC and aggregates
- IEX for charge variancy
- Reversed phase for multiple applications
- HILIC and HIC
COURSE OUTLINE
Introduction to Biomolecule Analysis
- Importance of biomolecule characterization in research and industry
- Challenges in HPLC-based biomolecule separation
- Amino Acids and Peptides
- Proteins and Their Structural Complexity
- Primary, secondary, tertiary, and quaternary structures
- Post-Translational Modifications (PTMs)
- Phosphorylation, glycosylation, disulfide bonds
- Monoclonal Antibodies (mAbs)
- Antibody-Drug Conjugates (ADC)
- Oligonucleotides and RNA-Based Therapeutics
- DNA, mRNA, and tRNA structures
- Transcription and translation processes
- Regulatory requirements for quality control and characterization
- Understanding Protein Aggregation and Its Impact
- SEC Column Selection and Mobile Phase Optimization
- Basic SEC Method Development for Biomolecule Purity Assessment
- Charge Variability in Biomolecules
- Modes of IEX: Cation vs. Anion Exchange
- Optimizing pH and Salt Gradients for Selective Separation
- Selection of Columns and Mobile Phases
- Separation of Proteins Based on Primary Structure
- Peptide Mapping and Characterization
- Derivatized Amino Acid Analysis Techniques
- Overview of HILIC and Its Applications
- Glycan Analysis and Carbohydrate Profiling
- Un-derivatized Amino Acid Analysis Using HILIC
- Fundamentals of HIC and Retention Mechanisms
- Common Applications in Protein and Antibody Analysis